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And, at the end of that study, In vivo, each rat still had liver enzyme levels that were within normal range! In another study (Hartgens, 1996), 16 bodybuilders using steroids were compared to 12 bodybuilders who were not. Then the bodybuilders who had used steroids stopped taking them for three months, at which points, the researchers found that liver enzymes had returned to the same levels as the non users. After only 3 months! We can see from the chart below that ex-steroid users have totally normal liver enzymes one year after they stop using. Let’s move on to some more useful studies. Take for example a 1995 study that showed the toxic effects of anabolic-androgenic steroids in primary rat hepatic cell cultures[3]. In this study the researchers used the following drugs and dosages: As proof of the hepatoxicity they used Lactate dehydrogenase release, neutral red retention, and glutathione depletion to determine plasma membrane damage, cell viability, and possible oxidative injury, respectively. What they showed was that the 17 alpha-alkylated steroids, methyltestosterone, stanozolol and oxymetholone, significantly increased Lactate dehydrogenase release and decreased neutral red retention at the 1×10^-4M dosage for 24h. Both methyltestosterone and oxymetholone also showed depleted glutathione at the 1×10^-4M dosage after 2h, 6h and 8h treatments. In other words they increased liver activity. You may also note that the other, non-alkylated steroids showed no significant difference in any levels. All in all this not only shows that 17 alpha-alkylated steroids are directly ‘hepatotoxic’, but also non-alkylated steroids are note hepatotoxic at all. But is this a real measure of hepatotoxicity? There is yet to be any correlation between the increase of the above-mentioned measurement and ‘hepatotoxicity’. Obviously, high dosages of the 17 alpha-alkylated steroids are potentially dangerous, but upon closer inspection, the study reveals more. Take a look, the researchers took cell cultures from the liversse of 60-day-old Sprague-Dawley rats. Not only are rat livers much smaller than human livers, but these were merely cultures. Furthermore, it was the 1×10^-4M concentrations that caused the most changes, but these are approximately 1 to a 1/3 of a full, daily human dosage — at least for the 17 alpha-alkylated steroids. Even at the 1×10^-6M concentration, there were no significant changes observed. It’s apparent that the levels of 17 alpha-alkylated steroids used were potentially toxic, but for a human to take the same amount would be insane. I’m guessing this could translate to maybe 4 grams every 24 hours or 28 grams a week if not more. Another study ( Boada 1999), attempted to show the acute and chronic effects of stanozolol on the liver. In acute treatments of stanozolol, dosages not mentioned, both cytochrome P456 and b5 (microsomal enzymes) levels dropped after 48 hours, and then at 72 hours, levels significant increased. On the other hand, with chronic treatments, time or dosage not mentioned, these microsomal enzymes showed a decrease in levels, how to order steroids in canada.
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